[HTML][HTML] At the crossroads of botanical collections and molecular genetics laboratory: a preliminary study of obtaining amplifiable DNA from moss herbarium material

M Saługa - PeerJ, 2020 - peerj.com
Background Research focused on extreme environments is often associated with difficulties
in obtaining fresh plant material. Herbaria may provide great support as they house large
collections of specimens from different parts of the world. Accordingly, there is also a
growing interest in methods using herbarium specimens in molecular studies. Much of the
literature on herbarium DNA is aimed to improve extraction and PCR amplification and is
focused mostly on vascular plants. Here, I provide a brief study of DNA extraction efficiency …

At the crossroads of botanical collections and molecular genetics laboratory: testing methods to obtain amplifiable DNA from moss herbarium material

M Saługa - 2019 - peerj.com
Background. Museum collections, including herbarium specimens, are considered an
invaluable source of DNA. They constitute a source of a precious commodity, particularly
when it is difficult to obtain living material of rare species, or extant populations occurred
only in hard to access geographical territories. It is apparent that herbaria should be directly
linked with molecular genetics laboratories making them a quick, open-source for molecular
projects. However, herbarium DNA is inherently characterised by high degradation and …
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